Physical Conditoins in Orion's Veil II: A Multi-Component Study of the Line of Sight Toward the Trapezium

Orion's Veil is an absorbing screen that lies along the line of sight to the Orion H II region. It consists of two or more layers of gas that must lie within a few parsecs of the Trapezium cluster. Our previous work considered the Veil as a whole and found that the magnetic field dominates the energetics of the gas in at least one component. Here we use high-resolution STIS UV spectra that resolve the two velocity components in absorption and determine the conditions in each. We derive a volume hydrogen density, 21 cm spin temperature, turbulent velocity, and kinetic temperature, for each. We combine these estimates with magnetic field measurements to find that magnetic energy significantly dominates turbulent and thermal energies in one component, while the other component is close to equipartition between turbulent and magnetic energies. We observe molecular hydrogen absorption for highly excited v, J levels that are photoexcited by the stellar continuum, and detect blueshifted S III and P III. These ions must arise from ionized gas between the mostly neutral portions of the Veil and the Trapezium and shields the Veil from ionizing radiation. We find that this layer of ionized gas is also responsible for He I absorption in the Veil, which resolves a 40-year-old debate on the origin of He I absorption towards the Trapezium. Finally, we determine that the ionized and mostly atomic layers of the Veil will collide in less than 85,000 years.Comment: 43 pages, 15 figures, to be published in Ap

Population genomics of the maize pathogen Ustilago maydis: demographic history and role of virulence clusters in adaptation

The tight interaction between pathogens and their hosts results in reciprocal selective forces that impact the genetic diversity of the interacting species. The footprints of this selection differ between pathosystems because of distinct life-history traits, demographic histories, or genome architectures. Here, we studied the genome-wide patterns of genetic diversity of 22 isolates of the causative agent of the corn smut disease, Ustilago maydis, originating from five locations in Mexico, the presumed center of origin of this species. In this species, many genes encoding secreted effector proteins reside in so-called virulence clusters in the genome, an arrangement that is so far not found in other filamentous plant pathogens. Using a combination of population genomic statistical analyses, we assessed the geographical, historical, and genome-wide variation of genetic diversity in this fungal pathogen. We report evidence of two partially admixed subpopulations that are only loosely associated with geographic origin. Using the multiple sequentially Markov coalescent model, we inferred the demographic history of the two pathogen subpopulations over the last 0.5 Myr. We show that both populations experienced a recent strong bottleneck starting around 10,000years ago, coinciding with the assumed time of maize domestication. Although the genome average genetic diversity is low compared with other fungal pathogens, we estimated that the rate of nonsynonymous adaptive substitutions is three times higher in genes located within virulence clusters compared with nonclustered genes, including nonclustered effector genes. These results highlight the role that these singular genomic regions play in the evolution of this pathogen

Feedback control of thermal lensing in a high optical power cavity

This paper reports automatic compensation of strong thermal lensing in a suspended 80 m optical cavity with sapphire test mass mirrors. Variation of the transmitted beam spot size is used to obtain an error signal to control the heating power applied to the cylindrical surface of an intracavity compensation plate. The negative thermal lens created in the compensation plate compensates the positive thermal lens in the sapphire test mass, which was caused by the absorption of the high intracavity optical power. The results show that feedback control is feasible to compensate the strong thermal lensing expected to occur in advanced laser interferometric gravitational wave detectors. Compensation allows the cavity resonance to be maintained at the fundamental mode, but the long thermal time constant for thermal lensing control in fused silica could cause difficulties with the control of parametric instabilities.This research was supported by the Australian Research Council and the Department of Education, Science and Training and by the U.S. National Science Foundation, through LIGO participation in the HOPF

Breakdown of electroneutrality in polyelectrolyte gels

Mathematical models of polyelectrolyte gels are often simplified by assuming the gel is electrically neutral. The rationale behind this assumption is that the thickness of the electric double layer (EDL) at the free surface of the gel is small compared to the size of the gel. Hence, the thin-EDL limit is taken, in which the thickness of the EDL is set to zero. Despite the widespread use of the thin-EDL limit, the solutions in the EDL are rarely computed and shown to match to the solutions for the electrically neutral bulk. The aims of this paper are to study the structure of the EDL and establish the validity of the thin-EDL limit. The model for the gel accounts for phase separation, which gives rise to diffuse interfaces with a thickness described by the Kuhn length. We show that the solutions in the EDL can only be asymptotically matched to the solutions for an electrically neutral bulk, in general, when the Debye length is much smaller than the Kuhn length. If the Debye length is similar to or larger than the Kuhn length, then phase separation can be initiated in the EDL. This phase separation spreads into the bulk of the gel and gives rise to electrically charged layers with different degrees of swelling. Thus, the thin-EDL limit and the assumption of electroneutrality only generally apply when the Debye length is much smaller than the Kuhn length

WISER Deliverable D3.3-2: The importance of invertebrate spatial and temporal variation for ecological status classification for European lakes

European lakes are affected by many human induced disturbances. In principle, ecological theories predict that the structure and functioning of benthic invertebrate assemblage (one of the Biological Quality Elements following the Water Framework Directive, WFD terminology) change in response to the level of disturbances, making this biological element suitable for assessing the status and management of lake ecosystems. In practice, to set up assessment systems based on invertebrates, we need to distiguish community changes that are related to human pressures from those that are inherent natural variability. This task is complicated by the fact that invertebrate communities inhabiting the littoral and the profundal zones of lakes are constrained by different factors and respond unevenly to distinct human disturbances. For example it is not clear yet how the invertebrates assemblages respond to watershed and shoreline alterations, nor the relative importance of spatial and temporal factors on assemblage dynamics and relative bioindicator values of taxa, the habitat constraints on species traits and other taxonomic and methodological limitations. The current lack of knowledge of basic features of invertebrate temporal and spatial variations is limiting the fulfillment of the EU-wide intercalibration of lake ecological quality assessment systems in Europe, and thus compromising the basis for setting the environmental objectives as required by the WFD. The aim of this deliverable is to provide a contribution towards the understanding of basic sources of spatial and temporal variation of lake invertebrate assemblages. The report is structured around selected case studies, manly involving the analysis of existing datasets collated within WISER. The case studies come from different European lake types in the Northern, Central, Alpine and Mediterranean regions. All chapters have an obvious applied objective and our aim is to provide to those dealing with WFD implementation at various levels useful information to consider when designing monitoring programs and / or invertebrate-based classification systems

A framework for the simulation of structural software evolution

This is the author's accepted manuscript. The final published article is available from the link below. Copyright @ 2008 ACM.As functionality is added to an aging piece of software, its original design and structure will tend to erode. This can lead to high coupling, low cohesion and other undesirable effects associated with spaghetti architectures. The underlying forces that cause such degradation have been the subject of much research. However, progress in this field is slow, as its complexity makes it difficult to isolate the causal flows leading to these effects. This is further complicated by the difficulty of generating enough empirical data, in sufficient quantity, and attributing such data to specific points in the causal chain. This article describes a framework for simulating the structural evolution of software. A complete simulation model is built by incrementally adding modules to the framework, each of which contributes an individual evolutionary effect. These effects are then combined to form a multifaceted simulation that evolves a fictitious code base in a manner approximating real-world behavior. We describe the underlying principles and structures of our framework from a theoretical and user perspective; a validation of a simple set of evolutionary parameters is then provided and three empirical software studies generated from open-source software (OSS) are used to support claims and generated results. The research illustrates how simulation can be used to investigate a complex and under-researched area of the development cycle. It also shows the value of incorporating certain human traits into a simulation—factors that, in real-world system development, can significantly influence evolutionary structures

Large Scale Flows from Orion-South

Multiple optical outflows are known to exist in the vicinity of the active star formation region called Orion-South (Orion-S). We have mapped the velocity of low ionization features in the brightest part of the Orion Nebula, including Orion-S, and imaged the entire nebula with the Hubble Space Telescope. These new data, combined with recent high resolution radio maps of outflows from the Orion-S region, allow us to trace the origin of the optical outflows. It is confirmed that HH 625 arises from the blueshifted lobe of the CO outflow from 136-359 in Orion-S while it is likely that HH 507 arises from the blueshifted lobe of the SiO outflow from the nearby source 135-356. It is likely that redshifted lobes are deflected within the photon dominated region behind the optical nebula. This leads to a possible identification of a new large shock to the southwest from Orion-S as being driven by the redshifted CO outflow arising from 137-408. The distant object HH 400 is seen to have two even further components and these all are probably linked to either HH 203, HH 204, or HH 528. Distant shocks on the west side of the nebula may be related to HH 269. The sources of multiple bright blueshifted Herbig-Haro objects (HH 202, HH 203, HH 204, HH 269, HH 528) remain unidentified, in spite of earlier claimed identifications. Some of this lack of identification may arise from the fact that deflection in radial velocity can also produce a change in direction in the plane of the sky. The best way to resolve this open question is through improved tangential velocities of low ionization features arising where the outflows first break out into the ionized nebula.Comment: Astronomical Journal, in press. Some figures are shown at reduced resolution. A full-resolution version is available at http://ifront.org/wiki/Orion_South_Outflows_Pape

Compensation of Strong Thermal Lensing in High Optical Power Cavities

In an experiment to simulate the conditions in high optical power advanced gravitational wave detectors such as Advanced LIGO, we show that strong thermal lenses form in accordance with predictions and that they can be compensated using an intra-cavity compensation plate heated on its cylindrical surface. We show that high finesse ~1400 can be achieved in cavities with internal compensation plates, and that the cavity mode structure can be maintained by thermal compensation. It is also shown that the measurements allow a direct measurement of substrate optical absorption in the test mass and the compensation plate.Comment: 8 page

Nuclear translocation of vitellogenin in the honey bee (Apis mellifera)

Vitellogenin (Vg) is a conserved protein used by nearly all oviparous animals to produce eggs. It is also pleiotropic and performs functions in oxidative stress resistance, immunity, and, in honey bees, behavioral development of the worker caste. It has remained enigmatic how Vg affects multiple traits. Here, we asked whether Vg enters the nucleus and acts via DNA-binding. We used cell fractionation, immunohistology, and cell culture to show that a structural subunit of honey bee Vg translocates into cell nuclei. We then demonstrated Vg-DNA binding theoretically and empirically with prediction software and chromatin immunoprecipitation with sequencing (ChIP-seq), finding binding sites at genes influencing immunity and behavior. Finally, we investigated the immunological and enzymatic conditions affecting Vg cleavage and nuclear translocation and constructed a 3D structural model. Our data are the first to show Vg in the nucleus and suggest a new fundamental regulatory role for this ubiquitous protein.Peer reviewe